parE [2019-08-01 14:31:10]

subunit of DNA topoisomerase IV

Locus: BSU_18090

Isoelectric point: 6.17

Molecular weight: 72.83 kDa

Protein length: 655 aa

Gene length: 1968 bp

Function: chromosome segregation and compaction

Product: subunit of DNA topoisomerase IV

Essential: yes

E.C.: 5.99.1.-

Synonyms: grlB

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA condensation/ segregation
• Groups of genes → Essential genes

Gene

Coordinates: 1,933,477 1,935,444

Phenotypes of a mutant

• essential PubMed
• upon overexpression, topoisomerase IV (ParE-ParC) can take over the functions of the type 1A topoisomerases TopA and TopB PubMed

The protein

Protein family

• type II topoisomerase GyrB family (together with GyrB) (according to UniProt)

Paralogous protein(s)

• GyrB

Domains

• TOPRIM domain (aa 423-537) PubMed

Structure

• 4I3H (from Streptococcus pneumoniae, 70% identity) PubMed

Localization

• Nucleoid (Homogeneous) PubMed
• uniformly distributed throughout the cell PubMed

Expression and Regulation

Operons

• Genes: parE-parC
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • LexA: repression, PubMed, in LexA regulon

  Regulation

  • induced by DNA damage (LexA) PubMed

  Additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of parE PubMed

Biological materials

LacZ fusion

• GP2550 (parE::lacZ cat, based on pAC5), available in Jörg Stülke's lab PubMed

References

Reviews

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Original publications

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